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adult healthy fresh human bone marrow aspirates  (Lonza)


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    Lonza adult healthy fresh human bone marrow aspirates
    Adult Healthy Fresh Human Bone Marrow Aspirates, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+human+bone+marrow+aspirates/human+bone+marrow+aspirates/pm38514887-384-8-20
    Average 90 stars, based on 1 article reviews
    adult healthy fresh human bone marrow aspirates - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Multifunctional Spider Silk Polymers for Gene Delivery to Human Mesenchymal Stem Cells
    Article Snippet: Briefly, an aspirate volume of 25mL was diluted 10-fold with MSC expansion medium consisting of DMEM:F12 basal medium (DMEM:F12) supplemented with 10% fetal bovine serum (FBS), antibiotics-antimycotics (100 U/mL penicillin, 100 μg/mL streptomycin, 0.25 μg/mL fungizone), 0.1 mM nonessential amino acids and 1 ng/mL basic fibroblast growth factor (bFGF) (Invitrogen). .. Cell Culture hMSCs were extracted according to common procedures [ 21 ] from commercially obtained fresh human bone marrow aspirates (Lonza, Basel, Switzerland). ..

    other:

    Article Title: Multifunctional Spider Silk Polymers for Gene Delivery to Human Mesenchymal Stem Cells
    Article Snippet: The amount of soluble formazan was measured at 490 nm using a microplate reader SpectraMax M2 (Molecular Devices, Sunnyvale, CA).



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    A Schematic of <t>human</t> <t>bone</t> <t>marrow</t> stromal cells (hBMSCs) isolation, expansion, and differentiation into human osteoblasts (hOBs) on DBP. After 2 weeks of remineralization in osteogenic differentiation medium, humanized RdBP was prepared by decellularization. B (i) hOBs adhere following the collagen fiber orientation on DBP but randomly on TCP. (ii) Alizarin red mineral staining after hOB culture on DBP and TCP. (iii) Time-course quantitative measurement of mineral deposition by fluorescent calcein staining ( n = 6 independent samples). C (i) Schematic of human CD14 + mononuclear cells (hCD14 + MNCs) isolation and expansion from peripheral blood using magnetic-activated cell sorting system. (ii) Brightfield images of ex vivo expanded hCD14 + cells for 1 week culture with hM-CSF. Representative images from 3 independent experiments. (iii) hCD14 + cells cultured on TCP, HP, and RdBP for 2 weeks with hM-CSF and hRANKL to induce hOC differentiation. D (i) Representative images of hOCs on TCP, HP, and RdBP. (green: phalloidin, blue: DAPI) (ii) Characterization of hOC sizes on TCP, HP, and RdBP after 2 weeks of the stimulated culture ( n = 20 cells from three independent experiments). E (i) Representative time-course fluorescent images of calcein-coated mineral resorption by hOCs on control, OS680-, and Zoledronate (Zol)-treated RdBP. (ii) Time-course quantitative monitoring of resorption areas. ( n = 3 independent samples) Inner panel images show mineral resorption (purple: OS680). F (i) Representative fluorescent images of hOCs on control, OS680-, and Zol-treated RdBP (green: phalloidin, blue: DAPI). (ii) Normalized hOC density at the end of 2 weeks culture ( n = 5 independent samples). The data are presented as mean ± standard deviation. P -values are derived from unpaired two-tailed t -tests. ns: not significant ( p > 0.05). Related source data are provided as a Source Data file.
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    A Schematic of <t>human</t> <t>bone</t> <t>marrow</t> stromal cells (hBMSCs) isolation, expansion, and differentiation into human osteoblasts (hOBs) on DBP. After 2 weeks of remineralization in osteogenic differentiation medium, humanized RdBP was prepared by decellularization. B (i) hOBs adhere following the collagen fiber orientation on DBP but randomly on TCP. (ii) Alizarin red mineral staining after hOB culture on DBP and TCP. (iii) Time-course quantitative measurement of mineral deposition by fluorescent calcein staining ( n = 6 independent samples). C (i) Schematic of human CD14 + mononuclear cells (hCD14 + MNCs) isolation and expansion from peripheral blood using magnetic-activated cell sorting system. (ii) Brightfield images of ex vivo expanded hCD14 + cells for 1 week culture with hM-CSF. Representative images from 3 independent experiments. (iii) hCD14 + cells cultured on TCP, HP, and RdBP for 2 weeks with hM-CSF and hRANKL to induce hOC differentiation. D (i) Representative images of hOCs on TCP, HP, and RdBP. (green: phalloidin, blue: DAPI) (ii) Characterization of hOC sizes on TCP, HP, and RdBP after 2 weeks of the stimulated culture ( n = 20 cells from three independent experiments). E (i) Representative time-course fluorescent images of calcein-coated mineral resorption by hOCs on control, OS680-, and Zoledronate (Zol)-treated RdBP. (ii) Time-course quantitative monitoring of resorption areas. ( n = 3 independent samples) Inner panel images show mineral resorption (purple: OS680). F (i) Representative fluorescent images of hOCs on control, OS680-, and Zol-treated RdBP (green: phalloidin, blue: DAPI). (ii) Normalized hOC density at the end of 2 weeks culture ( n = 5 independent samples). The data are presented as mean ± standard deviation. P -values are derived from unpaired two-tailed t -tests. ns: not significant ( p > 0.05). Related source data are provided as a Source Data file.
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    Image Search Results


    A Schematic of human bone marrow stromal cells (hBMSCs) isolation, expansion, and differentiation into human osteoblasts (hOBs) on DBP. After 2 weeks of remineralization in osteogenic differentiation medium, humanized RdBP was prepared by decellularization. B (i) hOBs adhere following the collagen fiber orientation on DBP but randomly on TCP. (ii) Alizarin red mineral staining after hOB culture on DBP and TCP. (iii) Time-course quantitative measurement of mineral deposition by fluorescent calcein staining ( n = 6 independent samples). C (i) Schematic of human CD14 + mononuclear cells (hCD14 + MNCs) isolation and expansion from peripheral blood using magnetic-activated cell sorting system. (ii) Brightfield images of ex vivo expanded hCD14 + cells for 1 week culture with hM-CSF. Representative images from 3 independent experiments. (iii) hCD14 + cells cultured on TCP, HP, and RdBP for 2 weeks with hM-CSF and hRANKL to induce hOC differentiation. D (i) Representative images of hOCs on TCP, HP, and RdBP. (green: phalloidin, blue: DAPI) (ii) Characterization of hOC sizes on TCP, HP, and RdBP after 2 weeks of the stimulated culture ( n = 20 cells from three independent experiments). E (i) Representative time-course fluorescent images of calcein-coated mineral resorption by hOCs on control, OS680-, and Zoledronate (Zol)-treated RdBP. (ii) Time-course quantitative monitoring of resorption areas. ( n = 3 independent samples) Inner panel images show mineral resorption (purple: OS680). F (i) Representative fluorescent images of hOCs on control, OS680-, and Zol-treated RdBP (green: phalloidin, blue: DAPI). (ii) Normalized hOC density at the end of 2 weeks culture ( n = 5 independent samples). The data are presented as mean ± standard deviation. P -values are derived from unpaired two-tailed t -tests. ns: not significant ( p > 0.05). Related source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Functional and analytical recapitulation of osteoclast biology on demineralized bone paper

    doi: 10.1038/s41467-023-44000-9

    Figure Lengend Snippet: A Schematic of human bone marrow stromal cells (hBMSCs) isolation, expansion, and differentiation into human osteoblasts (hOBs) on DBP. After 2 weeks of remineralization in osteogenic differentiation medium, humanized RdBP was prepared by decellularization. B (i) hOBs adhere following the collagen fiber orientation on DBP but randomly on TCP. (ii) Alizarin red mineral staining after hOB culture on DBP and TCP. (iii) Time-course quantitative measurement of mineral deposition by fluorescent calcein staining ( n = 6 independent samples). C (i) Schematic of human CD14 + mononuclear cells (hCD14 + MNCs) isolation and expansion from peripheral blood using magnetic-activated cell sorting system. (ii) Brightfield images of ex vivo expanded hCD14 + cells for 1 week culture with hM-CSF. Representative images from 3 independent experiments. (iii) hCD14 + cells cultured on TCP, HP, and RdBP for 2 weeks with hM-CSF and hRANKL to induce hOC differentiation. D (i) Representative images of hOCs on TCP, HP, and RdBP. (green: phalloidin, blue: DAPI) (ii) Characterization of hOC sizes on TCP, HP, and RdBP after 2 weeks of the stimulated culture ( n = 20 cells from three independent experiments). E (i) Representative time-course fluorescent images of calcein-coated mineral resorption by hOCs on control, OS680-, and Zoledronate (Zol)-treated RdBP. (ii) Time-course quantitative monitoring of resorption areas. ( n = 3 independent samples) Inner panel images show mineral resorption (purple: OS680). F (i) Representative fluorescent images of hOCs on control, OS680-, and Zol-treated RdBP (green: phalloidin, blue: DAPI). (ii) Normalized hOC density at the end of 2 weeks culture ( n = 5 independent samples). The data are presented as mean ± standard deviation. P -values are derived from unpaired two-tailed t -tests. ns: not significant ( p > 0.05). Related source data are provided as a Source Data file.

    Article Snippet: Fresh human bone marrow aspirate (50 mL) was purchased from Lonza (age 25–45 male and female donors).

    Techniques: Isolation, Staining, FACS, Ex Vivo, Cell Culture, Control, Standard Deviation, Derivative Assay, Two Tailed Test